The levels of hypoxia- and angiogenesis-related regulators and matrix metalloproteinase 9 activity in tear fluid of patients with non-penetrating ocular traumas

This article was focused on the evaluation of protein biomarkers related to thrombosis, hypoxia, angiogenesis, and tissue remodeling in tear fluid patients with non-penetrating corneal trauma. 32 injures were enrolled study, control group consisted 15 healthy patients. Samples collected from volunteers use a disposable end micropipette. Protein levels D-dimer, hypoxia-inducible factor 1α (HIF-1α), angiostatins, matrix metalloproteinase 9 (MMP-9) fluids determined by western blot analysis. Proteolytic activity values MMP-9 measured gelatin zymography. Results zymography assay calculated densitometry analysis expressed as arbitrary units. Significant increase D-dimer HIF-1α injured cornea 7.3 (p<0.05) 56 (p<0.001) folds, respectively shown, compared control, indicating thrombotic events hypoxia condition be involved pathogenesis ocular Dramatically elevated levels/activity enzyme (by 105 folds vs. p<0.001) suggests intense degradation extracellular damaged cornea. Up-regulation angiostatin level, products proteolytical cleavage plasminogen, traumatic eye comparison p<0.05), could represent an adaptive mechanism, which counteracts excessive hypoxia-induced neovascularization It is summarized that there strong association between elevation HIF-1α, suggesting thrombosis- hypoxia-mediated mechanisms triggering wound healing The findings this study are novel provide basis for further investigations reparation during Studied proteins can serve relevant appropriate diagnostic prognostic purposes.